SPHK1 AntibodyProduct information
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - Ca / cAMP / Lipid Signaling
SPHK1 Antibody #3297
|3297S||100 µl (10 western blots)||---||In Stock||---|
|3297||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||45||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
SPHK1 Antibody detects endogenous levels of total SPHK1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SPHK1. Antibodies are purified by peptide affinity chromatography.
Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.
- Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
- Xia, P. et al. (2000) Curr Biol 10, 1527-30.
- Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
- Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
- Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
- Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
- Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
- Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
- Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
- Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
- Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
- Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.