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Namocell


Single Cell Dispensers: The fastest way to a single cell

Company Profile

Namocell provides innovative single cell sorting and dispensing platforms to empower single cell research and therapeutics development. Namocell’s single cell dispensers are the fastest and easiest way to identify and isolate single cells, and enable users to accomplish single cell sorting and dispensing in one step in a way that is gentle to live cells. We serve researchers and scientists in a wide range of applications, including single cell genomics, cell line development, CRISPR, cell therapy, stem cell, monoclonal antibody development, rare cell isolation, and synthetic biology.

 


Effortless Single Cell Dispensing

  • Dispense single cells into 96-well plate in 1 min or 384-well plate in 6 min
  • Low pressure (<2 psi) preserves cell viability and integrity
  • Sort and dispense cells based on fluorescence and light scatter
  • Compatible with cell lines, primary cells, nuclei, bacteria, yeast and more
  • Starting sample can be as low as 100 cells
  • Isolate rare cell populations <0.1%
  • No clogging, no cross-contamination, zero maintenance

Namocell has combined the benefits of three key technologies: flow cytometry, microfluidics and liquid dispensing. The combination of these techniques is unique and enables users to accomplish single cell sorting and dispensing in one step, providing fast, efficient and gentle isolation of single cells.

Pala and Hana Single Cell Dispensers are capable of isolating single cells in a high throughput manner that is still gentle on cells so that they can maintain their viability. It is easy to operate and requires no prior experience or training with flow cytometry.

Namocell’s microfluidic cell cartridges ensure that there is no sample-to-sample cross contamination and keep the sample sterile. Cartridges that contain the sample material can be swapped out so there is no need for cleaning process.

Technology


Hana and Pala help you sort and dispense cells into 96-well or 384-well plates or 2mL tubes on
your own bench. Three patented sorting modes:

  • Single cell sorting mode dispenses single cells one-cell-per-well.
  • Enrichment mode isolates rare cells from extremely large samples.
  • Bulk sorting mode sorts tens of thousands cells in bulk


The Namo Single Cell Dispenser

Uses 2 or 3 fluorescence signals to sort and dispense single cells.

LEARN MORE ABOUT NAMO

The Hana Single Cell Dispenser

Uses 2 fluorescence signals and light scattering to sort and dispense single cells.

LEARN MORE ABOUT HANA

Unique Microfluidic Cell Cartridge

1. High Cell Viability, High Outgrowth Low sorting pressure (&lt;2 psi) preserves cell viability and integrity and delivers high outgrowth
2. Low Sample Input, High Recovery Near zero dead volume enables handling of small sample inputs down to 100 cells.
3. High Throughput Process 3M cells in 1 min and up to 150M cells/mL.
4. No Sample Carryover Disposable cell cartridge prevents cross-contamination between samples.
5. Safe – No Aerosol Formation 1 μL droplet prevents aerosol formation.

Unique Microfluidic Cell Cartridge

  • Low flow pressure preserves cell viability
  • Sorting and dispensing in a single stepprevents aerosol formation
  • No cross-contamination

LEARN MORE ABOUT DISPOSABLES



 

Application Notes

Namocell's Rare Cell Isolation: Rare cell isolation can be very challenging using conventional cell isolation methods when the target cell population frequencies are less than 1% such as circulating tumor cells (CTCs) in blood or low transfected GFP-positive cells within a pool. Although cell sorting is typically performed to isolate rare cells, this is not ideal due to factors that may compromise the rare cell viability and recovery including long sorting time, high pressure (typically more than 20 psi), and large dead volume.

Generating Clonal GFP Cell Lines: Generating a clonal GFP cell line typically requires first sorting GFP positive cells with a cell sorter. Then GFP positive cells are cultured for several days to recover from the high pressure damage caused by the cell sorter. Clonal GFP cells are finally obtained by manual limited dilution at 1 cell per well. In this application note, Namocell Single Cell Dispenser was used to directly isolate single GFP positive cells into 96-well cell culture plate. Due to the low pressure of the Namocell Single Cell Dispenser, clonal GFP cell lines can be generated in a single step.

B Cell Isolation for Single Cell Genomics: Single-cell RNA-sequencing (scRNA-seq) allows for dissection of gene expression profiles from individual cells, offering insights into sample heterogeneity and the genomic variability underlying molecular phenotypes. scRNA-seq begins with single cell isolation, which has traditionally been limited to expensive and complicated FACS instruments, often inconveniently housed in core facilities. Namocell’s single cell dispensers offer an alternative to FACS isolation, enabling rapid and reliable deposition of single cells for multi-omics approaches. In this application note, the Namo Single Cell dispenser was used to isolate single activated B cells labeled with both a cell viability dye and a fluorescent, cell type-specific marker.

Single cell isolation with Minimal Input: Obtaining a single cell is a critical step for single-cell transcriptomics, proteomics, and genomics. Traditional cell sorters have provided a powerful tool for sorting cells. However, it typically requires a large amount of cells. In this application note, Namocell Single Cell Dispenser was used to evaluate the single cell isolation efficiency of 100 minimal cell input with an adherent and non-adherent cell line.

Single Nucleus Dispensing: Single-cell RNA-sequencing (scRNA-seq) has been widely adopted for investigating gene expression in individual cells, offering unparalleled resolution into cell type-specific genomic identities. Recently, single nucleus RNA-sequencing (snRNA-seq) has emerged as a valuable alternative to scRNA-seq, allowing researchers to capture mRNA transcripts from individual nuclei. This approach is compatible with frozen cell or tissue samples and additionally reduces sample dissociation biases as well as dissociation-induced transcriptional stress responses. In this application note, a Namocell dispenser was used to isolate single DNA-labeled nuclei to demonstrate compatibility with snRNA-seq workflows.


Learn more about the Namocell Dispensers


BIOKÉ is the exclusive distributor of the Namocell in The Netherlands, Belgium and Luxembourg. Contact us if you have any questions about Namocell products or compatibility.

 

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