Product Pathways - Nuclear Receptor Signaling
RXRα (D6H10) Rabbit mAb #3085
|3085S||100 µl (10 western blots)||---||In Stock||---|
|3085||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||53||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
RXRα (D6H10) Rabbit mAb recognizes endogenous levels of total RXRα protein. This antibody does not cross-react with either RXRβ or RXRγ.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRα protein.
Western blot analysis of extracts from various cell lines using RXRα (D6H10) Rabbit mAb.
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human RXRα (hRXRα; +), RXRβ (hRXRβ; +), or RXRγ (hRXRγ; +), using RXRα (D6H10) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).
The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).
Retinoid X receptor α (RXRα) is the founding RXR family member and is predominantly expressed in the liver, kidney, epidermis, intestine, and a variety of tissues (2-4). Knockout of the murine rxrα gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXRα in retinoid signaling in vivo (5,6). Biochemical evidence suggests that RXRα transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade. MAPK-dependent phosphorylation of RXRα directly abrogates the ability of RXRα to associate with nuclear receptor coactivators (7).
- Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
- Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
- Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9.
- Dollé, P. et al. (1994) Mech Dev 45, 91-104.
- Kastner, P. et al. (1994) Cell 78, 987-1003.
- Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18.
- Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 7727 Biotinylated Protein Ladder Detection Pack
- 9999 Nonfat Dry Milk
- 2443 PPARγ (81B8) Rabbit mAb
- 2435 PPARγ (C26H12) Rabbit mAb
- 2554 RARα Antibody
- 8965 RARγ1 (D3A4) XP® Rabbit mAb
- 5388 RXRα Antibody
- 8715 RXRβ Antibody
- 5629 RXRγ Antibody
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 6348 SignalSilence® RXRα siRNA I
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.