DescriptionXhoI RE-Mix® is a ready to use 10X master mix combining the restriction enzyme, XhoI (NEB #R0146), NEBuffer, BSA and loading dye in a single tube. Simply add DNA and water and incubate for 15 minutes to perform the restriction digest.
Product SourceAn E. coli strain that carries the cloned XhoI gene from Xanthomonas holcicola (ATCC 13461)
Reaction Definition1X XhoI RE-Mix and DNA in a total reaction volume of 20 μl. Incubate at 37°C for 15 minutes.
One reaction is defined as the amount of RE-Mix that will digest 1 μg of DNA in 15 minutes at 37°C in a total reaction volume of 20 μl.
Properties and Usage
Heat Inactivation80°C for 20 min
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Impaired
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
- Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
- Ligation and Recutting (Terminal Integrity):
After an over-digestion of DNA with a restriction endonuclease the percentage of the DNA fragments ligated with T4 DNA ligase and the percentage that can be recut are determined by agarose gel electrophoresis.
- Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please contact NEB’s Global Business Development team at firstname.lastname@example.org.
- What are the advantages of using a RE-Mix Restriction Enzyme Master Mix?
- The bands on my gel are not as sharp as I would like. Is there a way to improve the sharpness?
- For some restriction enzymes star activity can be a concern. Is this also applicable to the RE-Mix?
- Is it possible to use RE-Mix in double digests?
- Can RE-Mix be used in overnight digestions?
- Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
- Why do I see additional DNA bands on my gel after a restriction digest?
- Why do I see a DNA smear on an agarose gel after a restriction digest?
- Why is my Restriction Enzyme not cutting DNA?
- How many nucletotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?