Product Pathways - Neuroscience
Stargazin (D33G3) Rabbit mAb #8511
|8511S||100 µl (10 western blots)||---||In Stock||---|
|8511||carrier free and custom formulation / quantity||email request|
|W||1:1000||Mouse, Rat||Endogenous||40||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Human.
Specificity / Sensitivity
Stargazin (D33G3) Rabbit mAb recognizes endogenous levels of total stargazin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human stargazin protein.
Stargazin is a four-pass transmembrane protein related to VDCC (voltage dependent calcium channel) γ subunits and part of the TARP (transmembrane AMPA receptor regulatory protein) family of proteins. TARP proteins can form a complex with AMPA receptors (GluR1-4) and serve as integral auxiliary subunits (1-6).
Interactions between stargazin and AMPA receptors are implicated in regulation of receptor surface expression, synaptic clustering and recycling, as well as increased receptor responsiveness to glutamate (1,2,5,6). Stargazin may play a role in the molecular mechanism of AMPAR-mediated inflammatory pain by taking part in signaling pathways that relay pain in the spinal cord (5). Because the protein also modulates the pharmacology of AMPA receptors, it enhances the effects of AMPAR potentiators that have therapeutic potential for a number of mental and neurodegenerative diseases (6).
The carboxy terminus of the stargazin protein interacts with the PDZ domains of PSD95 and other membrane-associated guanylate kinase (MAGUK) family members, and together traffic AMPA receptors to the cell surface membrane, anchoring them to the postsynaptic site (1,7). Phosphorylation of stargazin by PKA on Thr321 inhibits this binding (3).
- Tomita, S. et al. (2005) Nature 435, 1052-1058.
- Priel, A. et al. (2005) J. Neurosci. 25, 2682-2686.
- Choi, J. et al. (2002) J. Biol. Chem. 277, 12359-12363.
- Osten, P. and Stern-Bach, Y. (2006) Curr. Opin. Neurobiol. 16, 275-280.
- Tao, F. et al. (2006) J. Neurosci. Res. 84, 867-873.
- Tomita, S. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 10064-10067.
- Schnell, E. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 13902-13907.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 5306 AMPA Receptor (GluR 2) (D39F2) Rabbit mAb
- 4676 AMPA Receptor (GluR 3) (D47E3) Rabbit mAb
- 8070 AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb
- 7075 Anti-biotin, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 7727 Biotinylated Protein Ladder Detection Pack
- 5704 NMDAR1 (D65B7) Rabbit mAb
- 4212 NMDAR2B (D15B3) Rabbit mAb
- 9999 Nonfat Dry Milk
- 5842 PKA C-α (D38C6) Rabbit mAb
- 5675 PKA RI-α (D54D9) Rabbit mAb
- 8084 Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb
- 4027 Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody
- 3381 Phospho-NMDAR1 (Ser890) Antibody
- 5355 Phospho-NMDAR2B (Ser1284) Antibody
- 4208 Phospho-NMDAR2B (Tyr1472) Antibody
- 5661 Phospho-PKA C (Thr197) (D45D3) Rabbit mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.