E7335 FAQs for NEBNext Multiplex Oligos for Illumina (Index Primers Set 1), DNA Sets, Intl, NEB
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NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) FAQ

Q1: Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?
Q2: Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
Q3: Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
Q4: How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
Q5: Are these reagents available in a customized format? Larger format or 96 well format?

Q1: Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?

A1: Yes, NEBNext adaptor and primers for Illumina are validated by creating genomic DNA libraries as well as human mRNA libraries, followed by Illumina sequencing.


Q2: Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?

A2: Yes, NEBNext adaptor and primers for Illumina are compatible with NEBNext kits for Illumina DNA, mRNA and ChIP-Seq library preparation. However, they are not compatible with Illumina small RNA library preparation. For small RNA library preparation for Illumina, please refer to our kits (
NEB#E7300S/L) which contain their own adaptor and primers.


Q3: Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?

A3: Yes. The NEBNext adaptor for Illumina contains a specially modified nucleotide that needs to be cleaved by USER before PCR amplification.


Q4: How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?

A4: Up to 5 g of dA-tailed DNA fragments is recommended in a single ligation reaction with the NEBNext adaptor for Illumina.


Q5: Are these reagents available in a customized format? Larger format or 96 well format?

A5: Yes, customized formats can be accommodated. Please contact us at
oem@neb.com for more details.