Product Class: NEBNext

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Important! Please note that the concentration of the Universal PCR Primer and the Index Primers have changed from 25µM to 10µM
Catalog #SizeConcentration
E7335S24 reactions
E7335L96 reactions

Description











The NEBNext Multiplex Oligos for Illumina (Index Primers 1–12) contains adaptors and primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

Figure 1: Workflow
Figure 1: Workflow.
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext Adaptor for Illumina-20
USER Enzyme-20
NEBNext Universal PCR Primer for Illumina-20
NEBNext Index 1 Primer for Illumina-20
NEBNext Index 2 Primer for Illumina-20
NEBNext Index 3 Primer for Illumina-20
NEBNext Index 4 Primer for Illumina-20
NEBNext Index 5 Primer for Illumina-20
NEBNext Index 6 Primer for Illumina-20
NEBNext Index 7 Primer for Illumina-20
NEBNext Index 8 Primer for Illumina-20
NEBNext Index 9 Primer for Illumina-20
NEBNext Index 10 Primer for Illumina-20
NEBNext Index 11 Primer for Illumina-20
NEBNext Index 12 Primer for Illumina-20

Properties and Usage

Materials Required but not Supplied

  • Enzymes and buffers appropriate for DNA, CHiP or mRNA Library Preparation (NEB #E6000, E6040, E6056, E6100, E6110, E6200, E6240, E7420, E7445, E7530, E7370)
  • AMPure® XP Beads (Beckman Coulter, Inc.) or PCR Column Purification Kit (Qiagen or other)
  • Size Selection Materials [E-Gel® (Life Technologies, Inc.), Agarose Gel or AMPure XP Beads]
  • DNA LoBind® Tubes (Eppendorf®)/PCR Plates
  • Magnetic Stand
  • Bioanalyzer® (Agilent Technologies, Inc.)
  • Freshly Prepared 80% Ethanol
Please Refer to the Kit Specific Protocol for using the NEBNext Singleplex Oligos for Illumina:

The following kits are designed for use with the NEBNext Singleplex Oligos for Illumina:
  • #E7420, NEBNext Ultra Directional RNA Library Prep Kit for Illumina
  • #E7530, NEBNext Ultra RNA Library Prep Kit for Illumina
  • #E7370, NEBNext Ultra DNA Library Prep Kit for Illumina
  • #E7445, NEBNext Ultra Ligation Module
  • #E6040, NEBNext DNA Library Prep Master Mix Set for Illumina
  • #E6110, NEBNext mRNA Library Prep Master Mix Set for Illumina
  • #E6240, NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
  • #E6056, NEBNext Quick Ligation Module
  • #E6000, NEBNext DNA Library Prep Reagent Set for Illumina
  • #E6200, NEBNext ChIP-Seq Library Prep Reagent Set for Illumina
  • #E6100, NEBNext mRNA Library Prep Reagent Set for Illumina

Storage Temperature

-20°C

Supporting Documents

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
  1. Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?
  2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
  3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
  4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
  5. Are these reagents available in a customized format? Larger format or 96 well format?
  6. Can USER Enzyme be purchased separately?
  7. Are the NEBNext Oligos for Illumina compatible with single end and paired end sequencing?
  8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
  1. Please see manual (NEB #E7335) for protocols