Product Pathways - Ca / cAMP / Lipid Signaling
Annexin A1 (D16A10) Rabbit mAb #8691
|8691S||100 µl (10 western blots)||---||In Stock||---|
|8691||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Monkey, Pig||Endogenous||38||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Annexin A1 (D16A10) Rabbit mAb recognizes endogenous levels of total annexin A1 protein. This antibody does not cross-react with other members of the annexin family.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val236 of human annexin A1 protein.
The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).
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- Kim, S.W. et al. (2001) J Biol Chem 276, 15712-9.
- White, I.J. et al. (2006) EMBO J 25, 1-12.
- Varticovski, L. et al. (1988) Biochemistry 27, 3682-90.
- Dorovkov, M.V. and Ryazanov, A.G. (2004) J Biol Chem 279, 50643-6.
- Wang, W. and Creutz, C.E. (1994) Biochemistry 33, 275-82.
- Arur, S. et al. (2003) Dev Cell 4, 587-98.
- Perretti, M. and Gavins, F.N. (2003) News Physiol Sci 18, 60-4.
- Parente, L. and Solito, E. (2004) Inflamm Res 53, 125-32.
- Lim, L.H. and Pervaiz, S. (2007) FASEB J 21, 968-75.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Annexin V-FITC conjugate is a product manufactured for Cell Signaling Technology, Inc. by TAU Technologies BV.