E6270 FAQs for NEBNext® Fast DNA Library Prep Set for Ion Torrent, DNA Sets, Intl, NEB
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NEBNext® Fast DNA Library Prep Set for Ion Torrent™ FAQ

Q1: What sequencing platform can I use the NEBNext Fast DNA Library Prep Set for Ion Torrent reagents for?
Q2: What type of starting material can be used with NEBNext Fast DNA Library Prep Set for Ion Torrent?
Q3: How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Library Prep Set for Ion Torrent?
Q4: Does the NEBNext Fast DNA Library Prep Set for Ion Torrent work well for GC-rich DNA and AT-rich DNA?
Q5: Can I use the NEBNext Fast DNA Library Prep Set for Ion Torrent reagents for preparing RNA?
Q6: What methods for reaction clean up can be used with this kit?
Q7: What methods for size selection can be used with this kit?
Q8: Are these reagents available in a customized format or bulk format?

Q1: What sequencing platform can I use the NEBNext Fast DNA Library Prep Set for Ion Torrent reagents for?

A1: The NEBNext® Fast DNA Library Prep Set for Ion Torrent has been validated for the Ion Torrent™ Personal Genome Machine (PGM™ ).


Q2: What type of starting material can be used with NEBNext Fast DNA Library Prep Set for Ion Torrent?

A2: DNA that has previously been fragmented, by mechanical or enzyme-based shearing methods.


Q3: How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Library Prep Set for Ion Torrent?

A3: 100ng –1µg.


Q4: Does the NEBNext Fast DNA Library Prep Set for Ion Torrent work well for GC-rich DNA and AT-rich DNA?

A4: Yes. This product can be used with DNA comprised of a broad range of GC content.


Q5: Can I use the NEBNext Fast DNA Library Prep Set for Ion Torrent reagents for preparing RNA?

A5: Once the RNA has been converted to dsDNA, and fragmented to the appropriate size, these reagents can be used for the remainder of the sample preparation process.


Q6: What methods for reaction clean up can be used with this kit?

A6: Bead-based and column-based methods are compatible with this kit. Note that these reagents are not provided in this kit.


Q7: What methods for size selection can be used with this kit?

A7: This kit and protocol is compatible with size selection using gel elution (manual or automated). Note that these reagents are not provided in this kit.


Q8: Are these reagents available in a customized format or bulk format?

A8: Yes. Please contact us at
oem@neb.com for more details.