Product Pathways - Growth Factors/Cytokines
Human Lymphotoxin-α/TNF-β/TNFSF1 (hLT-α) #8230
|8230LC||50 µg (With Carrier)||---||In Stock||---|
|8230LF||50 µg (Carrier Free)||---||In Stock||---|
|8230SC||10 µg (With Carrier)||---||In Stock||---|
|8230SF||10 µg (Carrier Free)||---||In Stock||---|
Recombinant human LT-α (hLT-α) Pro36-Leu205 (Accession #NP_000586) was expressed in human 293 cells at Cell Signaling Technology.
Recombinant hLT-α contains no "tags" and the nonglycosylated protein has a calculated MW of 18,548. DTT-reduced and non-reduced protein migrate as 24-31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal PGVGL of recombinant hLT-α was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLT-α. All lots are greater than 98% pure.
The bioactivity of recombinant hLT-α was determined in an L-929 cell viability assay. The ED50 of each lot is between 15-150 pg/ml.
The purity of recombinant hLT-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLT-α and staining overnight with Coomassie Blue.
Less than 0.01 ng endotoxin/1 μg hLT-α.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLT-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Lymphotoxin-α (LT-α), also known as TNF–β, is a member of the TNF superfamily of proteins (1). NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-α (1). LT-α can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-β (LT α1β2 or LT α2β1) which can be cleaved from the cell surface by matrix metalloproteases (1,2). Soluble LT-α binds to and signals through TNFR1/TNFR2, activating the canonical NF-κB pathway (1). In contrast, LT α1β2 heterodimers bind to the LTβR receptor and activate the noncanonical NF-κB pathway (1). As a result, LT-α and TNF-α have overlapping functions. Soluble LT-α and LT α1β2 play key roles in lymphangiogenesis (3). The LT α1β2/LTβR axis is essential for the development of lymphoid tissue (1,3).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.