A suspension of E. coli K12 KS1000 which has been grown in M63 or M9 minimal glucose medium and brought to 50% glycerol. This strain is deficient in the Prc protease, a periplasmic protease (formerly known as Tsp, tail-specific protease). The Prc protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and presumably it can degrade proteins expressed in the periplasm as well.
F' lacIq lac+ pro+/ ara Δ(lac-pro) Δ(tsp)= Δ(prc)::KanReda51::Tn10(TetR) gyrA(NalR) rpoB thi-1 argE(am)
Properties and Usage
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.