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This strain is both Dam- and Dcm- , so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes (1,2). Suppresses many amber mutations when glutamine is acceptable but not the S100 or S7 mutations of λ, e.g., λgt11. Strain is identical to our previous Dam- Dcm- strain GM2163 except that the activity of nonspecific endonuclease I has been abolished, dramatically improving plasmid prep quality in many cases.
Woodcock, D. M. et al. (1989). Nucl.
Acids Res. 17, 3469-3478.
Palmer, B. R., and Marinus, M. G. (1994). Gene. 143, 1-12.