Product Class: Generic Enzyme

Histone H10 Human, Recombinant

Catalog #SizeConcentration
M2501S100 μg1 mg/ml

Description

Histone H1 acts on the linker region of polynucleosome DNA to condense the chromatin into structures of ~30 nm (1). It is not necessary for octamer or nucleosome core particle formation. Eight different Histone H1 proteins have been identified in the human genome (2). Histone H10 is a non replication-dependent histone that is highly expressed in cells that have terminally differentiated (3).

Protein Sequence:
TENST SAPAA KPKRA KASKK STDHP KYSDM IVAAI QAEKN RAGSS RQSIQ KYIKS HYKVG ENADS QIKLS IKRLV TTGVL KQTKG VGASG SFRLA KSDEP KKSVA FKKTK KEIKK VATPK KASKP KKAAS KAPTK KPKAT PVKKA KKKLA ATPKK AKKPK TVKAK PVKAS KPKKA KPVKP KAKSS AKRAG KKK (Genbank accession number: P07305)

ESI-TOF Analysis of Histone H10 Human, Recombinant.


SDS-PAGE analysis of Histone H10 Human, Recombinant.
Lane 1 and 7: NEB Protein Ladder (NEB #P7703), Lanes 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H10 Human, Recombinant.

Properties and Usage

Usage Concentration

1 mg/ml

Storage Temperature

-20°C

Storage Conditions

20 mM sodium phosphate
300 mM NaCl
1 mM EDTA
pH 7.0 @ 25°C

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Exonuclease Activity (Radioactivity Release):
    The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
  • Molecular Weight Determination (Mass Spectrometry) :
    The molecular weight of the product is determined using mass spectrometry.
  • N-terminal Protein Sequencing :
    Protein identity is confirmed using Edman Degradation to sequence the N-terminus of the intact protein.
  • Protease Activity (SDS-PAGE):

    The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE.

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. 1 mg/ml, 48 µM is calculated using the molar extinction coefficient for Histone H1 (3840) and its absorbance at 280 nm (4,5).  1.0 A280 units = 5.4 mg/ml.
  2. There is a small percentage of Histone H10 with a mass of 20863.27 which is a +131 Da difference from the major species. This correlates to Histone H10 with an intact N-terminal methionine (6).

References

  1. van Holde, K.E. (1989). Chromatin. 1
  2. Marzluff, W.F., et al. (2002). Genomics. 80, 487-497.
  3. Pehrson, J.R. and Cole, R.D. (1982). Biochem. 21, 456-460.
  4. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. . 182, 319-326.
  5. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
  6. Qing, X. et al. (2010). Biochemistry. 49, 5588-5599.
  1. Do the histones need to be reconstituted?
  2. What are the recommended histone storage conditions?
  3. Are the histones fusion proteins or tagged proteins?
  4. Can the histones be used as substrates for protein modification enzymes? Which ones?
After thawing on ice, mix well by pipetting the solution up and down. Do not centrifuge.