Product Class: Nucleic Acid Marker

TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

Catalog #SizeConcentrationGel Lanes
N3270S1.25 ml100 μg/ml250

Description

TriDye™ 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points.

TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.


N3270fig2_TN

2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane.

Properties and Usage

Bases

FragmentMassbp
14010002
2408001
3486001
4405001
5324001
61203001
7402017
8571517
9451200
101221000
1134900
1231800
1327700
1423600
15124500
1649400
1737300
1832200
1961100

Effective Size Range

100bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

0.006% Xylene Cyanol
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% bromophenol blue
0.06% Orange G
pH 7.9 @ 25°C

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  2. We recommend loading 5-10 μl (0.5-1.0 μg) of TriDye DNA ladder per gel lane.
  3. In general, it is recommended to load 1ul per mm of gel lane.
  4. TriDye DNA Ladder is stable for at least 6 months at 25°C.
  5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.
  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use SYBR® with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?

Selection Tools

Mix well before use, especially after thawing.