Product Class: Other

Therminator™ DNA Polymerase
NEBU cloned at NEB recombinant No

Product Introduction

Therminator DNA Polymerase is a 9°N™ DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides (1,2).

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Enhanced incorporation of modified nucleotides
  • Single base incorporation of dideoxy and acyclonucleotides
Catalog # Size Concentration
M0261S 200.0 units 2000 units/ml
M0261L 1000.0 units 2000 units/ml

Product Information

Description

Highlights

 


Product Source

An E. coli strain that carries the 9°N (D141A / E143A / A485L) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus species 9°N-7.
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X ThermoPol® Reaction Buffer

1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 90000 daltons

5' - 3' Exonuclease

No

3' - 5' Exonuclease

No

Strand Displacement

+

Unit Assay Conditions

1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed single-stranded M13mp18.

Application Features

  • DNA sequencing by partial ribosubstitution (3)
  • DNA sequencing using dideoxy (4) or acyclo (5) chain terminators
  • SNP analysis with dideoxy or acyclo chain terminators (6)

Product Notes

  1. Amplification of extended regions may require optimization of reaction conditions.

References

  1. Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2555.
  2. Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res. 30, 605-613.
  3. Barnes, W.F. (1978). J. Mol. Biol.. 119, 83-99.
  4. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
  5. Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
  6. Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What is the specific activity of Therminator™ DNA Polymerase?
  2. Can Therminator™ DNA Polymerase be used in other buffers?
  3. When should Therminator™ DNA Polymerase be used?
  4. Can Therminator™ DNA Polymerase be substituted for ThermoSequenase™?
  5. Can Therminator™ DNA Polymerase be used for sequencing?
  6. Why are some of the A's in my sequence weak when using Therminator™ DNA Polymerase?
  7. Does Therminator™ DNA Polymerase have 3'→5' proofreading exonuclease activity?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
9°N™ and THERMINATOR™ are trademarks of New England Biolabs, Inc.

SEQUENASE™ is a trademark of GE Healthcare.

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