Product Class: Nucleic Acid Marker

Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)

Catalog #SizeConcentrationGel Lanes
N0469S1.25 ml100 μg/ml250
N0469L3.75 ml100 μg/ml750

Description

Quick-Load® 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

N0469_thumb

Quick-Load 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane.

Properties and Usage

Bases

FragmentMassbp
14010,002
2408,001
3486,001
4405,001
5324,001
61203,001
7402,017
8571,517
9451,200
101221,000
1134900
1231800
1327700
1423600
15124500 / 517
1649400
1737300
1832200
1961100

Effective Size Range

100bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load 2-Log DNA Ladder per gel lane.
  3. In general, it is recommended to load 1µl per mm of gel lane.
  4. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  5. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
  6. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.
  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use SYBR® with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?

Selection Tools

Mix well before use, especially after thawing.