Product Class: Kit

Quick Blunting™ and Quick Ligation™ Kits

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

Product Introduction

NEB offers the Quick Blunting™ and Quick Ligation Kits™ together as a bundle at a value price.

  • The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. 
  • The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
Catalog # Size Concentration
E0542S 20.0 reactions
E0542L 100.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Protocol for the Quick Blunting Kit (E1201)
  2. Transformation Protocol
  3. Quick Ligation Protocol (M2200)

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. Will the PreCR Repair Mix blunt the ends of the DNA?

Troubleshooting