DescriptionThis mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate nonradioactive nucleotide in the reaction mixture. Use of synthetic d(N)6 primer ensures the presence of virtually all sequence combination of hexamer primers which results in equally labelled DNA of high specific activity (1,2). Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).
Sequence5' d(N6 ) 3' [N=A,C,G,T]
Properties and Usage
The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at email@example.com.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at firstname.lastname@example.org or fill out the Technical Support Form for appropriate document.
- For use in standard Random Priming Reaction, dissolve 33 µg of (NEB #S1230S) in 80 µl of water or 10X labeling buffer.
- 18.6 nmol of S1230 are supplied.
- Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 132
- Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 137
- Grunstein, M. and Hogness, D.S. Proc. Natl. Acad. Sci. USA. 72
- Southern, E.M. J. Mol. Biol.. 98
- Smith, G.E. and Summers, M.D. Anal. Biochem.. 109
- Hasse, A. et al. Methods Virol.. 7