R0708 FAQs for BaeGI, Restriction Endonucleases, Intl, NEB
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BaeGI FAQ

Q1: Is BaeGI a Time-Saver Qualified enzyme?
Q2: Is BaeGI activity sensitive to dam, dcm or mammalian CpG methylation?
Q3: How many base pairs should be added at the end of a PCR primer adjacent to the BaeGI recognition site to guarantee that BaeGI will cut properly?
Q4: Does BaeGI have any neoschizomers?

Q1: Is BaeGI a Time-Saver Qualified enzyme?

A1: Yes. BaeGI will digest 1 g of substrate DNA in 5 minutes using 1 l of enzyme under the recommended reaction conditions. For more information please
click here.


Q2: Is BaeGI activity sensitive to dam, dcm or mammalian CpG methylation?

A2: No. BaeGI is not sensitive to dam, dcm or mammalian CpG methylation. For up-to-date information about methylation sensitivities, please visit
REBASE.


Q3: How many base pairs should be added at the end of a PCR primer adjacent to the BaeGI recognition site to guarantee that BaeGI will cut properly?

A3: The addition of 6 base pairs is recommended.


Q4: Does BaeGI have any neoschizomers?

A4: No. For the most current information about isoschizomers/neoschizomers please refer to
REBASE.