R0636 FAQs for BfuCI, Restriction Endonucleases, Intl, NEB
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BfuCI FAQ

Q1: Is BfuCI activity sensitive to dam, dcm or mammalian CpG methylation?
Q2: How many base pairs should be added at the end of a PCR primer next to the BfuCI recognition site to guarantee that BfuCI will cut properly?
Q3: Does BfuCI have any isoschizomers?

Q1: Is BfuCI activity sensitive to dam, dcm or mammalian CpG methylation?

A1: Yes. BfuCI cannot cut recognition sites in mammalian gDNA that contain overlapping CpG methylation. For up-to-date information about methylation sensitivities, please visit
REBASE.


Q2: How many base pairs should be added at the end of a PCR primer next to the BfuCI recognition site to guarantee that BfuCI will cut properly?

A2: The addition of 6 base pairs is recommended.


Q3: Does BfuCI have any isoschizomers?

A3: Yes. DpnII, MboI and Sau3AI are isoschizomers of BfuCI. For the most current information about isoschizomers/neoschizomers please refer to
REBASE.