Protocols

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Traditional Cloning Quick Guide

Selection Charts

• Alphabetized List of Recognition Sequences
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Why Choose Recombinant Enzymes?

Web Tools

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

FAQs

1. Is BseYI activity sensitive to dam, dcm or mammalian CpG methylation?
2. How many base pairs should be added at the end of a PCR primer adjacent to the BseYI recognition site to guarantee that BseYI will cut properly?
3. Does BseYI have any isoschizomers?
4. When I run my sample in a gel after digesting with BseYI, the DNA doesn’t seem to migrate correctly. Is there a reason for that?
5. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

• Restriction Enzyme Troubleshooting Guide

Citations & Technical Literature