Product Class: Restriction Endonuclease

BseRI
neb31 cloned at NEB recombinant time saver dil_A 37 80 Heat

BseRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10184086. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GAGGAGNNNNNNNN_NN^
Isoschizomers | Single Letter Code

Product Introduction

  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Restriction Enzyme Cut Site: GAGGAG(10/8)
Catalog # Size Concentration
R0581S 200.0 units 5000 units/ml
R0581L 1000.0 units 5000 units/ml

Featured Videos

View Video Library
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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

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    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

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    Why is My Restriction Enzyme Not Cutting DNA?

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is extended digestion of BseRI recommended?
  2. Does BseRI have a single site in a common vector?
  3. What is the activity of BseRI at 25°C?
  4. Can over digestion be detrimental?
  5. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Tech Tips

This is a type IIS restriction enzyme which has binding protein properties. We don’t recommend using more than 10 units/ug or incubating for longer than 2 hours. Otherwise, degradation of the DNA may occur.

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