Product Class: Restriction Endonuclease

BcgI
neb31 cloned at NEB recombinant 2site dil_A 37 65 Heat CpG dam

SAM now included in enzyme formulation – no longer supplied as a separate vial.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNCGANNNNNNTGCNNNNNNNNNN_NN^

Product Introduction

  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.

  • Restriction Enzyme Cut Site: (10/12)CGANNNNNNTGC(12/10)

Catalog # Size Concentration
R0545S 250.0 units 2000 units/ml
R0545L 1250.0 units 2000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Why isn't BcgI cutting my DNA?
  2. Does BcgI require SAM for activity?
  3. Do BcgI generated ends ligate?
  4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting