Product Class: Restriction Endonuclease

AlwI
neb31 cloned at NEB recombinant dil_A 37 No dam

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GGATCNNNN^N_
Isoschizomers | Single Letter Code

Product Introduction

  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Restriction Enzyme Cut Site: GGATC(4/5)

Catalog # Size Concentration
R0513S 500.0 units 10000 units/ml
R0513L 2500.0 units 10000 units/ml

Featured Videos

View Video Library
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    Reduce Star Activity with High-Fidelity Restriction Enzymes

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    Standard Protocol for Restriction Enzyme Digests

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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

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    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

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    Why is My Restriction Enzyme Not Cutting DNA?

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

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    Double Digestion with NEBcloner

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    TIME-SAVER™ Protocol for Restriction Enzyme Digests

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    How to Prepare and Load a Standard DNA Ladder

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is AlwI blocked by dam methylation?
  2. Why is AlwI- cut DNA difficult to ligate?
  3. Why is an extended digest not recommended?
  4. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Tech Tips

This enzyme produces a single base extension, which is difficult to ligate.

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