Product Class: Restriction Endonuclease

PaeR7I
neb31 cloned at NEB recombinant time saver dil_A 37 No CpG

PaeR7I has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10145781. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB restriction endonuclease that recognizes the sequence C^TCGA_G
Isoschizomers | Single Letter Code

Product Introduction

  • Sourced from an E. coli strain that carries the PaeR7I gene from Pseudomonas aeruginosa PA0303 pMG7
  • Cleavage of mammalian genomic DNA is blocked by CpG methylation
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: C/TCGAG
Catalog # Size Concentration
R0177S 2000.0 units 20000 units/ml

Featured Videos

View Video Library
  • ReduceStarActivity_video_thumb

    Reduce Star Activity with High-Fidelity Restriction Enzymes

  • timesaverprotocol_video_thumb

    TIME-SAVER™ Protocol for Restriction Enzyme Digests

  • NEBTVEp15VLthumb280x210

    NEB® TV Ep. 15 – Applications of Restriction Enzymes

  • cuttingclose_video_thumb

    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

  • dnasmear_video_thumb

    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

  • EnzymeNotCutting_video_thumb

    Why is My Restriction Enzyme Not Cutting DNA?

  • TooManyBands_video_thumb

    Restriction Enzyme Digest Problem: Too Many DNA Bands

  • nebcloner_dd_demo_thumb

    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. How can this enzyme be inactivated?
  2. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  3. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Other Products You May Be Interested In