Product Class: Restriction Endonuclease

DpnI
neb31 recombinant time saver dil_B 37 80 Heat CpG Epi

DpnI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10196884. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

dpnIcutsite
Isoschizomers | Single Letter Code

Product Introduction

  • DpnI cleaves only when its recognition site is methylated.
  • Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation.
  • Methylation-sensitive restriction enzyme
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: GA/TC
Catalog # Size Concentration
R0176S 1000.0 units 20000 units/ml
R0176L 5000.0 units 20000 units/ml

Featured Videos

View Video Library
  • ReduceStarActivity_video_thumb

    Reduce Star Activity with High-Fidelity Restriction Enzymes

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    TIME-SAVER™ Protocol for Restriction Enzyme Digests

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    NEB® TV Ep. 15 – Applications of Restriction Enzymes

  • cuttingclose_video_thumb

    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

  • dnasmear_video_thumb

    Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel

  • EnzymeNotCutting_video_thumb

    Why is My Restriction Enzyme Not Cutting DNA?

  • TooManyBands_video_thumb

    Restriction Enzyme Digest Problem: Too Many DNA Bands

  • nebcloner_dd_demo_thumb

    Double Digestion with NEBcloner

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Will DpnI cleave hemimethylated DNA?
  2. What's the difference between DpnI, DpnII, MboI, and Sau3AI?
  3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  4. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  5. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  6. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

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