Product Class: Restriction Endonuclease

EcoRI
NEBU cloned at NEB recombinant time saver dil_C 37 65 Heat CpG

EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence G^AATT_C

Product Introduction

Catalog # Size Concentration
R0101S 10000.0 units 20000 units/ml
R0101T 10000.0 units 100000 units/ml
R0101L 50000.0 units 20000 units/ml
R0101M 50000.0 units 100000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is Star Activity and how can it be avoided?
  2. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  3. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?

Troubleshooting