Product Class: Nucleic Acid Marker

ϕX174 DNA-Hae III Digest

Catalog #SizeConcentrationGel Lanes
N3026S0.05 ml1,000 μg/ml50
N3026L0.25 ml1,000 μg/ml250

Description

The HaeIII digest of ΦX174 yields 11 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1).

Comes supplied with 1 vial of Gel Loading Dye, Blue (6X).



N3026_thumb

φX174 DNA-HaeIII Digest visualized by ethidium bromide staining. 1.7% agarose gel.

Properties and Usage

Bases

FragmentMassbp
12511,353
22001,078
3162872
4112603
558310
6a52281
6b50271
743234
836194
922118
101372

Effective Size Range

72bp to 1,353bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.
  2. 1X Gel Loading Dye, Blue:
    2.5% Ficoll-400
    11 mM EDTA
    3.3 mM Tris-HCl (pH 8.0@25°C)
    0.017% SDS
    0.015% bromophenol blue

References

  1. Fuchs, C. et al. (1978). Gene. 4, 1-23.
  2. Forster, A.C. et al. (1985). Nucl. Acids Res.. 13, 745-761.
  1. Why is the separation of the lower bands incomplete?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use SYBR® with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  1. Suggested protocol for loading a sample (N3026)
  2. Suggested protocol for loading a sample