Product Class: Other

λ DNA-Mono Cut Mix

Product Introduction

  • The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI.
  • The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing.
  • The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
  • For PFGE and standard electrophoresis.
  • Size range: 1,503 bp to 48,502 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Catalog # Size Concentration
N3019S 100.0 gel lanes (0.1 ml) 500 µg/ml

Protocols, Manuals & Usage

Protocols

  1. Suggested Loading Protocol for DNA Ladders & Markers

     

FAQs & Troubleshooting

FAQs

  1. How can I quantify the amount of DNA in each band of a marker?
  2. General information on Lambda DNA Mono Cut Mix:
  3. Can I use Midori Green with the DNA Ladders from NEB?
  4. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  5. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
  6. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.