Product Class: Other

Topoisomerase I (E. coli)
neb31 recombinant 37 65 Heat

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Product Introduction

  • Catalyzes the relaxation of negatively-supercoiled DNA
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
Catalog # Size Concentration
M0301S 100.0 units 5000 units/ml
M0301L 500.0 units 5000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Protocol for Topoisomerase I (E. coli) (M0301)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Does Topoisomerase I relax all supercoiled DNA to the same degree or are the products an equilibrium of various conformations?
  2. If the DNA resulting from a Topoisomerase I reaction is run in an ethidium bromide gel and there seems to be no evidence of enzyme activity, what is the most probable explanation?
  3. What is the molecular weight of Topoisomerase I?
  4. Can Topoisomerase I relax both positively and negatively supercoiled DNA?
  5. Does E. coli Toposiomerase I have the same properties as the Vaccinia Topoisomerase I?
  6. Will Topoisomerase I produce single strand-breaks in a plasmid?
  7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

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