Product Class: Other

RecJf
NEB2 cloned at NEB recombinant 37 65 Heat

Product Introduction

  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA in the 5' to 3' direction
  • Preferred substrate is double-stranded DNA with 5' single stranded overhangs > 6 nucleotides long
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

RecJf can be used for:

  • Degradation of single-stranded DNA from the 5'-end
  • Removal of 5' protruding single-stranded termini at the ends of linear double-stranded DNA (blunt ends are not exclusively created)
Catalog # Size Concentration
M0264S 1000.0 units 30000 units/ml
M0264L 5000.0 units 30000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Protocol for RecJf (NEB #M0264)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the activity of RecJf in the NEBuffers?
  2. Can DNA be blunted using RecJf?
  3. What is the difference between RecJf and Exonuclease I (NEB# M0293)?
  4. Can RecJf be heat inactivated?
  5. Will RecJF work in rCutSmart buffer?

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