Product Class: Other

Micrococcal Nuclease
NEBU recombinant 37 No ralbumin

Product Introduction

Micrococcal Nuclease is ideal for:

  • Preparation of double-stranded DNA fragments with 5'-OH and 3'-phosphate
  • Studies of chromatin structure
  • Degradation of nucleic acids in crude cell-free extracts
  • Preparation of rabbit reticulocyte

 

Catalog # Size Concentration
M0247S 320000.0 gel units 2000000 gel units/ml

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Will Micrococcal Nuclease cleave DNA as well as RNA and what type of end is left after digestion?
  2. Does Micrococcal Nuclease work in ChIP sequencing protocols?
  3. Will Micrococcal Nuclease work in rCutSmart Buffer?

Tech Tips

Addition of 1-5 mM Ca++ will increase activity if there is any EDTA or EGTA present
Addition of 1-5 mM EDTA or EGTA will chelate Ca++ and substantially decrease activity
Enzyme inhibited by higher than 100 mM NaCl
Used for chromatin structure analysis (CHiP)

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