Product Class: Other

dam Methyltransferase
NEBU recombinant 37 65 Heat SAM

M0222damMethyl_thumb

Product Introduction

  • Modifies the adenine residue (N6) of the sequence GATC
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
Catalog # Size Concentration
M0222S 500.0 units 8000 units/ml
M0222L 2500.0 units 8000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Recommended Protocol for 3H labeling of DNA
  2. Recommended Protocol for Methylation of Genomic DNA

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What should be considered if the methylation is not going to completion?
  3. Can dam Methyltransferase be heat inactivated?
  4. Does dam Methyltransferase require MgCl2?
  5. Is dam Methyltransferase sensitive to salt?
  6. Will DpnI cleave dam methylated DNA?
  7. Will any NEB methyltransferases (methylases) work on RNA?

Tech Tips

Always add the SAM to the reaction buffer just before doing the methylation reaction, because the SAM is unstable in an aqueous solution.

Keep the SAM frozen at -20C for a longer shelf life, fresh SAM is important for optimal methylation reactions.

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