Product Introduction

DNA Polymerase I, Large (Klenow) fragment was originally derived as a proteolytic product of E.coli DNA polymerase that retains polymerase and 3’ —> 5’ exonuclease activity

• Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
• Lacks 5’ —> 3’ exonuclease activity
• Generates probes using random primers
• Second strand cDNA synthesis