Product Class: Antibody

Anti-MBP Monoclonal Antibody (HRP conjugated)

Catalog #SizeConcentration
E8038S0.05 mg1 mg/ml
E8038L0.25 mg1 mg/ml

Description

Anti-MBP Monoclonal Antibody (HRP conjugated) is a murine anti-maltose binding protein antibody, isotype IgG2a. It is covalently linked to horseradish peroxidase and purified (1,2).

Properties and Usage

Antibody Type

Monoclonial

Isotype

Mouse Isotype IgG2a

Recommended Dilution for Western Blot

1:2000-1:5000

Storage Temperature

-20°C

Quality Control

Quality Assurance Statement

  • In an ELISA assay, a dilution of 1/2000 gives a signal of at least 20% of the maximum signal using concentrated antibody (detected with OPD as the substrate). The same 1/2000 dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. This antibody does not cross-react with other E. coli proteins.
  • In an ELISA assay, a dilution of 1/2000 gives a signal of at least 20% of the maximum signal using concentrated antibody (detected with OPD as the substrate). The same 1/2000 dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. This antibody does not cross-react with other E. coli proteins.

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM Thimerosal or an equivalent antimicrobial agent.

References

  1. Nakane, P. K. and Kawaoi, A. J. Histochem. Cytochem. 22.
  2. Boorsma, D. M. and Streefkerk, J. G. J. Histochem. Cytochem. 24.
  1. Western Transfer Protocol for Anti-MBP Monoclonal Antibody, HRP Conjugated