Product Class: Other

T7 Express Competent E. coli (High Efficiency)

Product Introduction

High efficiency chemically competent E. coli cells suitable for T7 protein expression.

  • T7 RNA Polymerase in the lac operon - no λ prophage
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Free of animal products
Catalog # Size Concentration
C2566H 20.0 x 0.05 ml
C2566I 6.0 x 0.2 ml

Protocols, Manuals & Usage

Protocols

  1. High Efficiency Transformation Protocol (C2566)
  2. 5 Minute Transformation Protocol (C2566)
  3. Protocol for Expression Using T7 Express (C2566)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What are the solutions/recipes (C2566)?
  2. What are the strain properties (C2566)?
  3. Why are there no colonies or no growth in liquid culture (C2566)?
  4. Why is there no protein visible on gel or no activity (C2566)?
  5. Why is induced protein insoluble (C2566)?
  6. Can I store competent cells at -20°C instead of -80°C?
  7. What is the difference between NEB #C2566H and NEB #C2566I?
  8. What is the optimal heat shock time for this strain (NEB #C2566H and NEB #C2566I)?
  9. How long should I incubate cells on ice after DNA has been added (NEB #C2566H and NEB #C2566I)?
  10. Is T7 Express (NEB #C2566H and NEB #C2566I) compatible with auto-induction procedures?
  11. Which kind of transformation tubes should be used?
  12. What volume of DNA can be added into competent cells?
  13. What is the shelf life for this strain (NEB #C2566H and NEB #C2566I)?
  14. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  15. What is the difference between T7 Express (NEB #C2566H/I) and BL21(DE3) (NEB #C2527H/I)?
  16. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  17. Is T7 expression subject to catabolite repression in NEB T7 Express or SHuffle strains?

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