DescriptionChemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC-series expression vector.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEB Yeast Transformation Reagent (5 ml)||4||1X|
Properties and Usage
- Ships on dry ice
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Sterility (K. lactis) :
The product is tested for bacterial and/or fungal contamination by a plating assay.
- Transformation Efficiency:
The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
LicensesNotice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.
- Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.
- Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.
- Plates containing colonies can be stored at 4°C for up to two weeks.
Always store immediately at -80 C. Yeast competent cells will quickly lose competency if handled improperly.