DescriptionThis medium pack includes 12 grams of Yeast Carbon Base and 10 ml of Acetamide.
The Yeast Carbon Base (YCB) Medium Pack contains sufficient reagents needed to make 1 liter of YCB Agar Medium containing 5 mM acetamide.
YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
- Yeast Carbon Base Medium (12g)
- Acetamide solution (sterile) (10 ml)
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Yeast Carbon Base Medium (12g)||4|
|Acetamide solution (sterile) (10 ml)||4||100X|