DescriptionNew England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of protein kinases, including Abl Protein Tyrosine Kinase (Abl) (NEB# P6050), CDK1-cyclin B (NEB# P6020), CaMKII (NEB #P6060), p42 MAP Kinase (MAPK) (NEB# P6080).
Properties and Usage
0.01% Brij 35
Quality Assurance Statement
- This reaction buffer is free of detectable protease and phosphatase activities.
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Phosphatase Activity (PNPP):
The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation there is no detectable phosphatase activity as determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.
- Protease Activity (SDS-PAGE):
The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE.