Product Class: Reaction Buffer

Exoglycosidase Reaction Buffer Pack

Catalog #SizeConcentration
B0719S5 x 1 ml

Description

New England Biolabs supplies a 10X reaction buffer with all of its enzymes.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
G1 Reaction Buffer10X
G2 Reaction Buffer10X
G4 Reaction Buffer10X
G6 Reaction Buffer10X
G7 Reaction Buffer10X

Properties and Usage

Storage Temperature

-20°C

Quality Control

Quality Assurance Statement

  • These buffers are free of detectable protease, exoglycosidase and endoglycosidase activities.

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. Buffer Compositions

    1X G1 Reaction Buffer:
    50 mM Sodium Citrate
    pH 6.0 @ 25°C

    1X G2 Reaction Buffer:
    50 mM Sodium Citrate
    pH 4.5 @ 25°C
     
    1X G4 Reaction Buffer:
    50 mM Sodium Citrate
    100 mM NaCl
    pH 6.0 @ 25°C
     
    1X G6 Reaction Buffer:
    5 mM CaCl2
    50 mM Sodium Acetate
    pH 5.5 @ 25°C

    1X G7 Reaction Buffer:
    50 mM Sodium Phosphate
    pH 7.5 @ 25°C
  1. Can I use α1-3,6 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?
  2. Can I use β-N-Acetylglucosaminidase in a double digest with other exoglycosidases and/or endoglycosidases?
  3. Can I use β1-4 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?