Product Pathways - MAPK Signaling
MEK1 (61B12) Mouse mAb #2352
|2352S||100 µl (10 western blots)||---||In Stock||---|
|2352||carrier free and custom formulation / quantity||email request|
|W||1:2000||Human, Mouse, Rat, Monkey||Endogenous||45||Mouse IgG2a|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
MEK1 (61B2) Mouse mAb detects endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full length MEK1 proteins.
Western blot analysis of extracts from HeLa, NIH/3T3 and A431 cells, using MEK1 (61B2) Mouse mAb.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® MEK1 siRNA I #6426 or SignalSilence® MEK1 siRNA II #6530 (+), using MEK1 (61B12) Mouse mAb #2352 and α-Tubulin (11H10) Rabbit mAb #2125. The MEK1 (61B12) Mouse mAb confirms silencing of MEK1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of MEK1 siRNA.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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