Product Pathways - Neuroscience
Huntingtin (D7F7) XP® Rabbit mAb #5656
|5656S||100 µl (10 western blots)||---||In Stock||---|
|5656P||40 µl (4 western blots)||---||In Stock||---|
|5656||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||350||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Huntingtin (D7F7) XP® Rabbit mAb detects endogenous levels of total huntingtin protein. Species cross-reactivity for IHC-P is in rodent only.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1220 of human huntingtin protein.
Western blot analysis of extracts from rat and mouse brain tissue using Huntingtin (D7F7) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain using Huntingtin (D7F7) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Huntingtin (D7F7) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat brain using Huntingtin (D7F7) XP® Rabbit mAb.
Huntingtin Disease (HD) is a fatal neurodegenerative disorder characterized by psychiatric, cognitive, and motor dysfunction. Neuropathology of HD involves specific neuronal subpopulations: GABA-ergic neurons of the striatum and neurons within the cerebral cortex selectively degenerate (1,2). The genetic analysis of HD has been the flagship study of inherited neurological diseases from initial chromosomal localization to identification of the gene.
Huntingtin is a large (340-350 kD) cytosolic protein that may be involved in a number of cellular functions such as transcription, gastrulation, neurogenesis, neurotransmission, axonal transport, neural positioning, and apoptosis (2,3). The HD gene from unaffected individuals contains between 6 and 34 CAG trinucleotide repeats, with expansion beyond this range causing the onset of disease symptoms. A strong inverse correlation exists between the age of onset in patients and the number of huntingtin gene CAG repeats encoding a stretch of polyglutamine peptides (1,2). The huntingtin protein undergoes numerous post-translational modifications including phosphorylation, ubiquitination, sumoylation, palmitoylation, and cleavage (2). Phosphorylation of Ser421 by Akt can partially counteract the toxicity that results from the expanded polyglutamine tract. Varying Akt expression in the brain correlates with regional differences in huntingtin protein phosphorylation; this pattern inversely correlates with the regions that are most affected by degeneration in diseased brain (2). A key step in the disease is the proteolytic cleavage of huntingtin protein into amino-terminal fragments that contain expanded glutamine repeats and translocate into the nucleus. Caspase mediated cleavage of huntingtin at Asp513 is associated with increased polyglutamine aggregate formation and toxicity. Phosphorylation of Ser434 by CDK5 protects against cleavage (2,3).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.