Product Pathways - Protein Translation
SKAR α/β (D65E8) XP® Rabbit mAb #5439
|5439S||100 µl (10 western blots)||---||In Stock||---|
|5439P||40 µl (4 western blots)||---||In Stock||---|
|5439||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||46, 43||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SKAR α/β (D65E8) XP® Rabbit mAb detects endogenous levels of total SKAR protein, α and β isoforms. By western blot, the antibody detects an approximately 21 kDa band of unknown origin in all primate cell types tested.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human SKAR.
Western blot analysis of extracts from various cell types using SKAR α/β (D65E8) XP® Rabbit mAb.
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.