GST (91G1) Rabbit mAb (HRP Conjugate) #5475
|5475S||100 µl (10 western blots)||---||In Stock||---|
|5475||carrier free and custom formulation / quantity||email request|
|W||1:1000||Transfected Only||Rabbit IgG|
Applications Key: W=Western Blotting
Specificity / Sensitivity
GST (91G1) Rabbit mAb (HRP Conjugate) detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (91G1) Rabbit mAb #2625.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
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