Product Pathways - Tyrosine Kinase / Adaptors
EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb #2085
|2085S||100 µl (10 western blots)||---||In Stock||---|
|2085||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-P=Immunofluorescence (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb detects endogenous levels of EGFR E746-A750del mutant protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.
Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper), and total EGFR control antibody (lower).
Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).
Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).
Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb.
The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.
- Simonetti, S. et al. (2010) J Transl Med 8, 135. Applications: IF-P (paraffin).
- Brevet, M. et al. (2010) J Mol Diagn 12, 169-76. Applications: IHC-P (paraffin).
- Kawahara, A. et al. (2010) Clin Cancer Res , . Applications: IHC-P (paraffin), Western Blotting.
- Yu, J. et al. (2009) Clin Cancer Res 15, 3023-8. Applications: IF-P (paraffin), IHC-P (paraffin), Western Blotting.
- Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57. Applications: IHC-P (paraffin).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 4405 EGF Receptor (15F8) Rabbit mAb
- 2646 EGF Receptor (C74B9) Rabbit mAb
- 4267 EGF Receptor (D38B1) XP® Rabbit mAb
- 3197 EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb
- 2963 EGF Receptor (T43) Antibody
- 3265 EGF Receptor (V279) Antibody
- 2232 EGF Receptor Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 8112 SignalStain® Antibody Diluent
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The manufacture, use, sale and import of this product is within the scope of one or more intellectual property rights (including patents and patent applications) owned or controlled by Cell Signaling Technology. The purchase of this product conveys to the buyer a non-transferrable right to use the purchased product only in research conducted by the buyer. The sale of the product is expressly conditioned on the buyer not using the products or its components (1) to analyze or reverse engineer the product for its chemical/physical properties and composition (including e.g., identification of the sequence); (2) in manufacturing; (3) to provide a service, information, or data to an unaffiliated third party for payment; (4) for therapeutic, diagnostic or prophylactic purposes; (5) resale, whether or not such product are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Cell Signaling Technology, Inc. Business Development at firstname.lastname@example.org.