Product Pathways - Metabolism
DHCR24/Seladin-1 (C59D8) Rabbit mAb #2033
|2033S||100 µl (10 western blots)||---||In Stock||---|
|2033||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||54||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
DHCR24/Seladin-1 (C59D8) Rabbit mAb detects endogenous levels of total DHCR24/Seladin-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human DHCR24/Seladin-1.
Western blot analysis of extracts from various cell types using DHCR24/Seladin-1 (C59D8) Rabbit mAb.
DHCR24/Seladin-1 was identified as a molecular basis for desmosterolosis (1). It encodes for 24-dehydrocholesterol reductase (3β-hydroxysterol Δ-24-reductase). This enzyme reduces desmosterol in cholesterol biosynthesis (1). Recessive mutations in this gene in desmosterolosis patients lead to a defective enzyme resulting in increased levels of desmosterol (1). DHCR24/Seladin-1 is induced upon oxidative stress and was found to mediate Ras-induced senescence resulting from increased reactive oxygen species (2). Studies further indicate that the level of DHCR24/Seladin-1 is induced in the acute response and reduced in the chronic response to oxidative stress in a cholesterol dependent manner (3). Moreover, overexpression of DHCR24/Seladin-1 bearing two mutations that abolish its reductase acitivity causes the cells to lose protection from oxidative stress (3). These findings thus link the reductase activity of DHCR24/Seladin-1 to its protective role in oxidative stress. This enzyme has also been demonstrated to be a hydrogen peroxide scavenger (4).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
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