BioFire Defense BCHM-ASY-0005 LCGreen® Plus+ Melting Dye
LCGreen dye is specifically designed for high-resolution melting curve analysis to detect DNA sequence variants. The addition of LCGreen dye increase the melting temperature of DNA by 1-3 °C and may requre adjustment of cycling parameters. This dye is manufactured exclusively by BioFire Defense and its chemical structures are unique among the scientific and patent literature.
LCGreen dye is tailored specifically for Hi-Res Melting and has the unique ability to detect heteroduplexes during melting analysis after PCR. Just add the dye to your sample before PCR. LCGreen dye is extremely stable, does not inhibit PCR, and is a "saturation" dye which makes it possible to detect multiple PCR products in a mixture during melting analysis.
LCGreen Plus+ is tailored for use in melting instruments with 96- or 384-well microtiter plates. It has superb fluorescence intensity and can be used with other fluorescence based PCR detection systems. For optimal performance, the use of a Hi-Res melting instrument is required.
For enhanced ease of use, a complete PCR Master Mix is available from BioFire Defense Inc. The LightScanner Master Mix is an optimized PCR master mix, which includes LCGreen Plus.
Conventional dsDNA dyes cannot be used at high concentrations due to dye redistribution during melting curve analysis.
Saturation of dsDNA binding sites eliminates potential for dye redistribution during melting curve acquisition.
|Technical note||Hi-Res Melting For Mutation Scanning Using LCGreen Plus Dye||download|
|Brochure / Flyer||LCGreen Plus+||download|
|MSDS||MSDS Sheet - LCGreen I and Plus||download|