BioFire Defense HRLS-ASY-0002 LightScanner Master Mixes
LightScanner master mix brilliantly provides a pre-optimized solution specifically designed to deliver superior PCR performance for Hi-Res Melting® and genotyping applications.
This master mix reduce time and saves money with easy-to-use premixed formulas. This secures downstream success by directly going from PCR to melting curve analysis and eliminate the risk of contamination.
LightScanner Master Mix
This master mix incorporates a Taq polymerase, an antibody-based hot-start method, dNTPs, magnesium chloride, and BioFire Defenses proprietary LCGreen® PLUS dye and is formulated at a 2.5X concentration. The antibody-mediated hot-start method means no enzyme pre-activation is required before or during thermocycling, translating into much faster PCR protocol times than other methods of hot-start protection.
The master mix includes a concentration of magnesium that is optimized to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high GC amplicons.
|1 x 100 Rxn
|| 2.5X Master Mix
||10 mM MgCI2
||Reagent Grade Water
||LightScanner Master Mix User's Guide
- Reduce time, save money - easy-to-use premixed formula, at 2.5x concentration, limits steps and need for optimization.
- Secure downstream success - specifically designed for LightScanner applications, the master mix includes LCGreen Plus dye enabling you to go directly from PCR to melting curve analysis, eliminating the risk of contamination
|Brochure / Flyer||LCGreen Plus+||download|
|Brochure / Flyer||LightScanner Master Mix||download|
|Technical note||LightScanner® Hi-Res Melting® Comparison of Six Master Mixes for Scanning and Small Amplicon and LunaProbes™ Genotyping||download|
|MSDS||LightScanner Master Mix||download|
|Manual||LightScanner Master Mix Users Guide - 100 and 500 Reaction Kit||download|