Product Pathways - MAPK Signaling
PhosphoPlus® Elk-1 (Ser383) Antibody Kit #9180
|9180S||1 Kit (10 western blots)||---||In Stock||---|
|9180||carrier free and custom formulation / quantity||email request|
|Kit Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-Elk-1 (Ser383) Antibody #9181||100 µl||W||H||H, M, R, Mk, Z, Dg||47 kDa||Rabbit|
|Elk-1 Antibody #9182||100 µl||W||H, M, R, Z||47 kDa||Rabbit|
|Elk-1 Control Proteins #9183||150 µl||40 kDa nonphosphorylated, 50 kDa phosphorylated kDa||E. Coli|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||W||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||W||All||Goat|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each||W|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting
Reactivity Key: H=Human, M=Mouse, R=Rat, Z=Zebrafish, All=All Species Expected
Western blot analysis of Elk-1 fusion protein phosphorylated in vitro with purified Erk2 enzyme, using control Elk-1 Antibody.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody (upper) or control Elk-1 Antibody #9182 (lower).
The PhosphoPlus® Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Elk-1. Bacterially expressed Elk-1 serves as a negative control. The same protein phosphorylated by p42 MAP Kinase (Erk2) serves as a positive control.
Specificity / Sensitivity
Elk-1 Antibody detects endogenous levels of total Elk-1 protein. Phospho-Elk-1 (Ser383) Antibody detects EIk-1 protein only when activated by phosphorylation at serine 383.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Elk-1, or with a synthetic peptide derived from the sequence of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
- Aplin, A. E. et al. (2001) J. Biol. Chem. 153, 273-282. Applications: IC-ABC, Western Blotting.
- Hu, Y. et al. (2000) J. Immunol. 165, 2142-2418. Applications: Western Blotting.
- Wang, D. and Richmond, A. (2001) J. Biol. Chem. 276, 3650-3659. Applications: Western Blotting.
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- 7727 Biotinylated Protein Ladder Detection Pack
- 9182 Elk-1 Antibody
- 9183 Elk-1 Control Proteins
- 9184 Elk-1 Fusion Protein
- 9186 Phospho-Elk-1 (Ser383) (2B1) Mouse mAb
- 9181 Phospho-Elk-1 (Ser383) Antibody
- 9100 PhosphoPlus® p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Kit
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.