Product Pathways - MAPK Signaling
PhosphoPlus® Elk-1 (Ser383) Antibody Kit #9180
|9180S||1 Kit (10 western blots)||---||In Stock||---|
|9180||carrier free and custom formulation / quantity||email request|
|Kit Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-Elk-1 (Ser383) Antibody #9181||100 µl||W||H||H, M, R, Mk, Z, Dg||47 kDa||Rabbit|
|Elk-1 Antibody #9182||100 µl||W||H, M, R, Z||47 kDa||Rabbit|
|Elk-1 Control Proteins #9183||150 µl||40 kDa nonphosphorylated, 50 kDa phosphorylated kDa||E. Coli|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||W||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||W||All||Goat|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each||W|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting
Reactivity Key: H=Human, M=Mouse, R=Rat, Z=Zebrafish, All=All Species Expected
Western blot analysis of Elk-1 fusion protein phosphorylated in vitro with purified Erk2 enzyme, using control Elk-1 Antibody.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody (upper) or control Elk-1 Antibody #9182 (lower).
The PhosphoPlus® Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Elk-1. Bacterially expressed Elk-1 serves as a negative control. The same protein phosphorylated by p42 MAP Kinase (Erk2) serves as a positive control.
Specificity / Sensitivity
Elk-1 Antibody detects endogenous levels of total Elk-1 protein. Phospho-Elk-1 (Ser383) Antibody detects EIk-1 protein only when activated by phosphorylation at serine 383.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Elk-1, or with a synthetic peptide derived from the sequence of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
- Aplin, A. E. et al. (2001) J. Biol. Chem. 153, 273-282. Applications: IC-ABC, Western Blotting.
- Hu, Y. et al. (2000) J. Immunol. 165, 2142-2418. Applications: Western Blotting.
- Wang, D. and Richmond, A. (2001) J. Biol. Chem. 276, 3650-3659. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.