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NucleoSpin RNA Plant

Product information

Benefits

  • Efficient removal of contaminating DNA – rDNase included for on-column digestion*
  • Efficient sample homogenization and reduction of viscosity –  NucleoSpin® Filters (shredders) included
  • Up to 70 μg ready-to-use RNA
  • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Plant system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size

Code Name Size Quantity Price
740949.10
NucleoSpin RNA Plant
10 preps    - Unavailable in your region
740949.50
NucleoSpin RNA Plant
50 preps    - Unavailable in your region
740949.250
NucleoSpin RNA Plant
250 preps    - Unavailable in your region
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